Yoshihiro Shimizu
- Areas of expertise
- Professor Yoshihiro Shimizu is the Japan project lead for ACROPATH. He developed the PURE system for cell free protein synthesis which will be integral to the Acropath project
- Location
- Japan: RIKEN
Yoshihiro Shimizu is a Team Director in RIKEN Center for Biosystems Dynamics Research (BDR). He developed a reconstituted cell-free protein synthesis system (PURE system) in his Ph.D. research in the University of Tokyo. After supporting the commercialization of the PURE system in a startup company, he became an assistant professor at the University of Tokyo and continued his research for the development of applied techniques using the PURE system. In 2010, he started his own research group in RIKEN which aims to further apply the cell-free system for the field of synthetic biology and proteomics study.
Key references:
ePURE_JSBML: A tool for constructing a deterministic model of a reconstituted Escherichia coli protein translation system with a user-specified nucleic acid sequence.
Shimizu Y., Tanimura N., and Matsuura T.
Advanced Biology 7: e2200177 (2023).
DOI: 10.1002/adbi.202200177
Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs.
Hibi K., Amikura K., Sugiura N., Masuda K., Ohno S., Yokogawa T., Ueda T., and Shimizu Y.
Communications Biology 3: 350 (2020).
DOI: 10.1038/s42003-020-1074-2
In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.
Shimojo M., Amikura K., Masuda K., Kanamori T., Ueda T., and Shimizu Y.
Communications Biology 3: 142 (2020).
DOI: 10.1038/s42003-020-0874-8